Highly sensitive and specific qualitative molecular test using Real-Time Polymerase Chain Reaction (Real-Time PCR) technique to detect DNA of Herpes Simplex Virus Type 2 (HSV-2). Performed on samples from genital or anal infection sites (vesicle fluid, ulcer swab, cervical/urethral swab) or body fluids. Ideal standard for diagnosing active infection and differentiating between HSV-1 and HSV-2 in genital area.