17-Hydroxycorticosteroid (17-OHCS) analysis is a quantitative measurement of a group of steroid hormone metabolites containing a hydroxyl group at carbon 17, typically performed on a 24-hour urine sample. This group primarily includes cortisol metabolites (corticosteroid sugar hormones) and androgen precursor metabolites (such as 11-deoxycortisol). This test is traditionally considered an indicator of the overall activity of the adrenal cortex in producing glucocorticoid hormones.