1,25-Dihydroxyvitamin D (1,25-Dihydroxyvitamin D) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a precise test that measures the level of active vitamin D in the body. Vitamin D is an essential vitamin that plays a vital role in regulating calcium and phosphorus levels, affecting bone health and the immune system. This test is used to diagnose disorders such as thyroid insufficiency, malabsorption conditions, and to assess patients’ response to vitamin D therapy. It is highly accurate and provides valuable information about the level of active vitamin D, helping doctors develop appropriate treatment plans.