An advanced LC-MS/MS test measuring active Vitamin D, providing highly accurate results to evaluate calcium metabolism and bone health in complex clinical cases.
Trusted medical contentUpdated: 25-04-2026Views: 86LHL-116
Medical notice: this information is for education and service understanding. It is not a diagnosis or a recommendation for an unnecessary procedure.
About this service
1,25-Dihydroxyvitamin D (1,25-Dihydroxyvitamin D) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a precise test that measures the level of active vitamin D in the body. Vitamin D is an essential vitamin that plays a vital role in regulating calcium and phosphorus levels, affecting bone health and the immune system. This test is used to diagnose disorders such as thyroid insufficiency, malabsorption conditions, and to assess patients’ response to vitamin D therapy. It is highly accurate and provides valuable information about the level of active vitamin D, helping doctors develop appropriate treatment plans.
Purpose Test
Active Vitamin D levels: Measures the active form of vitamin D in the body, which plays a key role in regulating calcium and phosphorus levels.
Thyroid disorders: Helps in diagnosing conditions such as hyperthyroidism or hypothyroidism.
Malabsorption issues: Can reveal problems in vitamin absorption from the intestines, such as Crohn’s disease or celiac disease.
Bone formation status: Used to assess bone health and ensure the body receives adequate vitamin D to support bone formation.
Patient response to treatment: Can be used to monitor patient response to vitamin D therapy and adjust doses accordingly.
Recommended groups
Individuals with vitamin D deficiency symptoms: such as persistent fatigue, bone pain, and muscle weakness.
Patients with thyroid disorders: to assess active vitamin D levels in cases of hyperthyroidism or hypothyroidism.
Individuals with malabsorption conditions: such as Crohn’s disease or celiac disease, which affect the body’s ability to absorb vitamins.
People receiving vitamin D therapy: to monitor treatment effectiveness and adjust doses as needed.
Individuals at risk of osteoporosis: such as the elderly or those with a family history of osteoporosis.
Pregnant women: to evaluate vitamin D levels and support maternal and fetal health.
Patients with chronic health conditions: such as kidney failure or diabetes, as vitamin D levels may be affected.
Symptoms indicative
Persistent fatigue or unexplained tiredness.
Bone or joint pain.
Muscle weakness or difficulty in movement.
Growth problems in children or delayed development.
Mood changes: such as depression or anxiety.
Calcium deficiency signs: such as muscle cramps or numbness.
Frequent infections: indicating a weakened immune system.
Dental problems: such as tooth decay or weak gums.
Normal Levels
between 15 and 60 pmol/L.
What is included?
✓1,25-Dihydroxyvitamin D (Liquid Chromatography-Tandem Mass Spectrometry - LC-MS/MS) Test
✓The optimal health guidance based on the result.
✓Personal support services from Lahalak
Conditions and notices
Fasting is not required
The terms and conditions announced on the "Lahalak" medical platform apply.
There are no special conditions for the service other than those mentioned.
Frequently Asked Questions
Short answers to the FAQs published with this service.
What is the 1,25-Dihydroxyvitamin D test?
It is a test that measures the level of active vitamin D in the body, which plays an important role in regulating calcium and phosphorus levels.
Why should I take this test?
It is usually performed to diagnose vitamin D deficiency, assess thyroid disorders, or monitor patient response to vitamin D treatment.
How is this test performed?
A blood sample is drawn by a healthcare professional, usually from a vein.
Is any special preparation required before the test?
Service resources and references
References and additional resources
Reference material and files associated with this service when available.
Scientific references
Jones, G., Kaufmann, M., & Thai, B. (2015). Quantification of 1,25‑dihydroxyvitamin D2 and D3 in serum using liquid chromatography‑tandem mass spectrometry. Clinical Chemistry and Laboratory Medicine.
(Describes a sensitive LC‑MS/MS method quantifying both 1,25‑OH2D2 and 1,25‑OH2D3 after immunoaffinity extraction.)
Taylor, P. N., et al. (2015). Determination of human reference values for serum total 1,25‑dihydroxyvitamin D using an extensively validated 2D ID‑UPLC‑MS/MS method. Clinical Chemistry and Laboratory Medicine.
(Provides reference ranges based on precise LC‑MS/MS measurement of active vitamin D metabolites.)
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Content on LahaLak is provided for educational purposes only. It is not a medical diagnosis, prescription, or substitute for consulting a qualified clinician. Medical decisions should not be made from this content alone.
Active Vitamin D Test Price | Lahalak Medical Platform
It generally does not require special preparation, but it is best to consult the doctor for specific instructions.
What are the normal levels of active vitamin D in the blood?
Normal levels vary by laboratory, but they are generally between 15 and 60 pmol/L.
What are the risks associated with this test?
The test is generally safe, but mild pain or bruising at the blood draw site may occur.
What happens if the test results are abnormal?
Results should be discussed with a doctor, who may recommend further testing or treatment plans based on vitamin D levels.
How can vitamin D deficiency or excess be treated?
Treatment depends on the condition and may include vitamin supplements, dietary adjustments, or medical consultation for causes of excess.
Smith, J. A., & Chan, N. (2015). Quantitation of 1α,25‑dihydroxyvitamin D by LC‑MS/MS using solid‑phase extraction and fixed‑charge derivatization, compared to immunoextraction methods. Clinical Chemistry and Laboratory Medicine.
(Compares analytical performance of LC‑MS/MS methods with different sample preparation techniques.)
Lee, H., & Patel, R. (2023). Overcome isomer interference in 1α,25‑dihydroxyvitamin D quantitation by liquid chromatography‑tandem mass spectrometry. Journal of Applied Laboratory Medicine.
(Discusses advanced LC‑MS/MS strategies to improve specificity by addressing interfering vitamin D isomers.)
