The focus of free thyroxine (FREE T4) is measured in serum, which is unlined by blood proteins (such as albums and TBG) of total thyroxine (T4). FT4 represents the active physiological form of biology capable of entering cells and organizing metabolism (metabolism). FT4 is a more reliable indicator of the actual thyroid function of T4 measurement, where the changes are not affected by the levels of proteins