Chlamydia trachomatis DNA testing by PCR from urine is a highly sensitive and specific molecular test. It aims to detect the DNA of the bacterium Chlamydia trachomatis in a first-void urine (FVU) sample. DNA amplification techniques such as polymerase chain reaction (PCR) or isothermal DNA amplification assays (e.g., SDA, TMA) are used. It is the preferred method for routine screening in both males and females due to the ease of sample collection and its high accuracy.