This test measures total Vitamin D levels, essential for calcium absorption, bone strength, and supporting the immune system against various health conditions.
Trusted medical contentUpdated: 25-04-2026Views: 99LHL-114
Medical notice: this information is for education and service understanding. It is not a diagnosis or a recommendation for an unnecessary procedure.
About this service
Vitamin D (25 OH-Vit D -Total) test is a blood test that measures the level of active vitamin D in the body. Vitamin D is essential for maintaining healthy bones and teeth, as it helps in the absorption of calcium and phosphorus. It also plays an important role in supporting the immune system, promoting heart health, and reducing the risk of certain chronic diseases. This test is usually performed to assess vitamin D levels in individuals who show deficiency symptoms such as weak bones, joint pain, or fatigue. It is also recommended for people at risk of vitamin D deficiency, such as the elderly, those living in areas with limited sunlight, or those following a vegetarian diet. Based on the test results, the doctor may recommend taking vitamin D supplements or making dietary changes to ensure adequate levels of this essential vitamin.
Purpose Test
Vitamin D deficiency: The test can detect a vitamin D deficiency, which may lead to bone problems such as osteoporosis or rickets, and muscle weakness.
Calcium and phosphorus-related disorders: Helps evaluate the body’s ability to absorb calcium and phosphorus necessary for healthy bones and teeth.
Immune system issues: Vitamin D deficiency may indicate a weakened immune system and increased susceptibility to infections.
Chronic diseases: Low vitamin D levels can be associated with a higher risk of heart disease, diabetes, and certain types of cancer.
Recommended groups
People with symptoms of vitamin D deficiency: such as bone pain, muscle weakness, chronic fatigue, or weakened immunity.
Elderly individuals: as the ability to produce vitamin D from sunlight decreases with age, increasing the risk of deficiency.
People with limited sun exposure: such as those living in areas with long winters or working indoors all day.
Individuals with darker skin: because darker skin absorbs less sunlight, which may reduce vitamin D production.
Pregnant or breastfeeding women: to ensure adequate vitamin D levels for the health of both mother and child.
People with malabsorption disorders: such as Crohn's disease or celiac disease, which can impair vitamin absorption.
Individuals with bone diseases or chronic health issues: such as osteoporosis or autoimmune disorders.
People following a strict vegan diet: as some plant-based diets lack natural sources of vitamin D.
Symptoms indicative
Bone pain: Experiencing bone pain or weakness may indicate vitamin D deficiency, leading to conditions such as rickets or osteoporosis.
Muscle weakness: Weak muscles or persistent physical fatigue can be a sign of vitamin D deficiency.
Chronic fatigue: Feeling continuously tired despite adequate sleep may result from vitamin D deficiency.
Mood disorders: Vitamin D deficiency may cause mood swings or contribute to depression.
Weakened immune system: Frequent infections or seasonal illnesses may indicate vitamin D deficiency, which plays a role in immune function.
Slow wound healing: Vitamin D deficiency can affect the body’s ability to heal wounds or injuries.
Hair loss: Abnormal hair loss may be associated with vitamin D deficiency.
Growth and development issues in children: Vitamin D deficiency in children may lead to poor growth or delayed bone development.
Normal Levels
Vitamin D Deficiency: Less than 20 ng/mL or less than 50 nmol/L.
Mild Vitamin D Deficiency: Between 20 to 29 ng/mL or 50 to 74 nmol/L.
Normal Levels: From 30 to 50 ng/mL or 75 to 125 nmol/L.
Vitamin D Excess: More than 50 ng/mL or more than 125 nmol/L, which may indicate vitamin D excess.
Vitamin D deficiency can lead to health problems such as osteoporosis or muscle weakness.
Normal levels are essential to maintain bone health and immune system function.
Excess vitamin D may cause high calcium levels in the blood, potentially leading to kidney or heart issues.
If results show deficiency or excess, a doctor may recommend lifestyle adjustments or supplements based on the situation.
What is included?
✓25 OH-Vit D -Total Test
✓The optimal health guidance based on the result.
✓Personal support services from Lahalak
Conditions and notices
Fasting is not required
The terms and conditions announced on the "Lahalak" medical platform apply.
There are no special conditions for the service other than those mentioned.
Frequently Asked Questions
Short answers to the FAQs published with this service.
What is the Vitamin D test?
It is a blood test that measures the active form of vitamin D in the body and determines whether its levels are within the normal range or if there is a deficiency or excess.
When should the Vitamin D test be done?
It is recommended if you have symptoms of vitamin D deficiency such as fatigue, bone pain, muscle weakness, or if you belong to groups at risk of deficiency.
How is the test performed?
A small blood sample is taken from a vein in the arm and sent to the laboratory to analyze vitamin D levels.
Does the test require fasting?
Service resources and references
References and additional resources
Reference material and files associated with this service when available.
Scientific references
Chen, H., McCoy, L. F., Schleicher, R. L., & Pfeiffer, C. M. (2008). Measurement of serum 25‑hydroxyvitamin D2 and 25‑hydroxyvitamin D3 using liquid chromatography‑tandem mass spectrometry and comparison to a radioimmunoassay method. Clinical Chemistry and Laboratory Medicine. (Describes an LC‑MS/MS method for 25(OH)D2 and 25(OH)D3 in human serum and compares it with traditional immunoassay techniques.)
Liebisch, G., et al. (2015). Accurate and reliable quantification of 25‑hydroxyvitamin D species in serum by LC‑MS/HR‑MS with reference material alignment. Journal of Lipid Research. (Presents a fast and accurate high‑resolution LC‑MS/MS assay for multiple 25(OH)D analytes and discusses analytical specificity and standardization.)
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Content on LahaLak is provided for educational purposes only. It is not a medical diagnosis, prescription, or substitute for consulting a qualified clinician. Medical decisions should not be made from this content alone.
No, the vitamin D test does not require fasting and can be done at any time of the day.
What are the normal levels of vitamin D in the blood?
Normal levels range from 30 to 50 ng/mL. Less than 20 ng/mL is considered deficient, while over 50 ng/mL may indicate excess.
What causes vitamin D deficiency?
Limited sun exposure, poor nutrition, malabsorption issues, and some chronic diseases such as kidney disorders.
How can vitamin D deficiency be treated?
Treatment usually involves taking vitamin D supplements, adequate sun exposure, and consuming foods rich in vitamin D such as fatty fish and fortified milk.
How often should the test be done?
Testing frequency is recommended based on the doctor’s advice, especially for at-risk groups or those receiving treatment for deficiency.
Can there be excess vitamin D?
Yes, excessive intake of vitamin D supplements can lead to excess, which may cause health issues such as elevated blood calcium levels.
Cavalier, E., Bhattoa, H. P., Heijboer, A. C., Lombardi, G., Delatour, V., Van Uytfanghe, K., Makris, K., Jørgensen, N. R., Herrmann, M., Pikner, R., Lukas, P., & Vasikaran, S. D. (2025). Assessment of measurement uncertainty of immunoassays and LC‑MS/MS methods for serum 25‑hydroxyvitamin D. Clinical Chemistry and Laboratory Medicine, 63(10), 1981–1991. (Evaluates and compares analytical performance of LC‑MS/MS and immunoassay methods for measuring serum total 25(OH)D.)
Jenkinson, C., et al. (2016). High throughput LC‑MS/MS method for simultaneous determination of 25‑hydroxyvitamin D metabolites in human serum. Journal of Lipid Research. (Discusses LC‑MS/MS as a reference method for serum 25(OH)D measurement with superior specificity over immunoassays.)
