Primary use: pre-transfusion compatibility testing (Crossmatch) to detect any irregular antibodies in the recipient’s blood that may attack donor red blood cells, preventing serious hemolytic reactions. Testing pregnant women (especially Rh-negative) to detect formation of antibodies against fetal blood antigens (e.g., anti-D), which may cause hemolytic disease of the newborn (HDN) in the current or future pregnancies. Testing individuals who have previously received blood transfusions, as prior exposure may stimulate production of irregular antibodies. Investigating potential causes of hemolytic anemia, alongside the direct Coombs test.

All patients requiring blood or blood component transfusions (e.g., surgery, bleeding, severe anemia, etc.). All pregnant women as a routine test in the first trimester. Patients with a history of adverse reactions after blood transfusion. Individuals with a history of recurrent miscarriages or unexplained fetal deaths. Individuals with a history of recurrent miscarriages or unexplained fetal deaths.

There are no direct symptoms, as it is a routine preventive test. It is mandatory before any surgical procedure that may require a blood transfusion. It is requested as part of routine prenatal care. It is requested as part of routine prenatal care.

The normal result is negative, meaning no detectable irregular antibodies are present in the patient’s serum against the standard test cell panel. A positive result is reported with identification of the detected antibody (e.g., anti-D, anti-Kell, anti-E). The antibody titer may also be reported, especially in pregnancy, as a higher titer indicates an increased risk of fetal involvement.

Indirect Coombs Test Price | Lahalak Medical Platform

A vital screening to detect irregular antibodies in the serum, essential for ensuring blood compatibility before transfusion and safely monitoring high-risk pregnancies and fetal health.

Scientific name: Indirect Coombs Test

An immunological test aimed at detecting the presence of free and unbound IgG antibodies (and sometimes IgM) in the patient’s serum or plasma. These antibodies are known as "irregular antibodies" because they are not naturally present in everyone. The test is performed by mixing the patient’s serum with red blood cells of known antigens, then addin...

Service type Laboratory test (Blood)

Duration 10-15 minutes

Fasting Fasting is not required

Included Services

Coombs Test (Indirect)
Consultation for results interpretation with a hematology or blood bank specialist.
Personal support services from Lahalak

Medical Service Information

Purpose Test

Primary use: pre-transfusion compatibility testing (Crossmatch) to detect any irregular antibodies in the recipient’s blood that may attack donor red blood cells, preventing serious hemolytic reactions.
Testing pregnant women (especially Rh-negative) to detect formation of antibodies against fetal blood antigens (e.g., anti-D), which may cause hemolytic disease of the newborn (HDN) in the current or future pregnancies.
Testing individuals who have previously received blood transfusions, as prior exposure may stimulate production of irregular antibodies.
Investigating potential causes of hemolytic anemia, alongside the direct Coombs test.

Recommended groups

All patients requiring blood or blood component transfusions (e.g., surgery, bleeding, severe anemia, etc.).
All pregnant women as a routine test in the first trimester.
Patients with a history of adverse reactions after blood transfusion. Individuals with a history of recurrent miscarriages or unexplained fetal deaths.
Individuals with a history of recurrent miscarriages or unexplained fetal deaths.

Symptoms indicative

There are no direct symptoms, as it is a routine preventive test.
It is mandatory before any surgical procedure that may require a blood transfusion. It is requested as part of routine prenatal care.
It is requested as part of routine prenatal care.

Frequently Asked Questions (FAQs)

Indirect (IAT): Detects free antibodies in plasma before they can attach to red blood cells. It answers the question: "Does your blood have weapons (antibodies) ready to attack foreign blood?" Used for prevention.
Direct (DAT): Detects antibodies already attached to red blood cells after a reaction has occurred. It answers the question: "Is your immune system attacking your red blood cells now?" Used for diagnosis.
The antibody must be accurately identified to know which antigen it targets.
Blood provided to the patient in the future must be free of that antigen if transfusion is needed.
During pregnancy, the fetus must be closely monitored if the father carries that antigen.

Normal Levels

The normal result is negative, meaning no detectable irregular antibodies are present in the patient’s serum against the standard test cell panel.
A positive result is reported with identification of the detected antibody (e.g., anti-D, anti-Kell, anti-E). The antibody titer may also be reported, especially in pregnancy, as a higher titer indicates an increased risk of fetal involvement.

Conditions & Notices

• Fasting is not required.
• The sample is collected in a serum tube (red cap) or a plasma tube (purple cap with EDTA).
• The physician and laboratory should be informed of any previous blood transfusions, pregnancies, or miscarriages.
• For patients requiring a blood transfusion: the test is performed on a recent sample (usually within 72 hours before transfusion) to ensure no new antibodies have developed during this period.