The Vitamin B2 (Riboflavin) by HPLC test is a laboratory procedure used to measure the levels of riboflavin (Vitamin B2) in the blood. Vitamin B2 is an essential nutrient that plays a critical role in energy metabolism, as well as the health of the skin and eyes. A blood sample is collected and sent to a laboratory, where it is analyzed using **High-Performance Liquid Chromatography (HPLC)**. This technique provides precise and accurate measurements of riboflavin levels in the blood. This test is recommended in cases of suspected riboflavin deficiency or when symptoms such as fatigue, weakness, or skin problems are present. The results help doctors assess the patient’s nutritional status and determine if supplementation or dietary adjustments are necessary to ensure healthy levels of Vitamin B2, supporting overall health and well-being.