Testosterone Total analysis is a laboratory test used to measure the level of the hormone testosterone in the blood. Testosterone is an essential hormone that plays a central role in the development of male sexual characteristics, as well as influencing muscle mass, bone density, and energy levels. This test is performed to evaluate testosterone levels in both men and women and is often requested in cases of hormonal imbalance, such as decreased libido, erectile dysfunction, or infertility in men. It is also used to assess conditions such as polycystic ovary syndrome in women, where testosterone levels may be elevated. A blood sample is usually collected from a vein, preferably in the morning when hormone levels are at their peak. Test results are interpreted based on reference ranges that may vary slightly between laboratories, so it is always recommended to consult a specialist physician to determine whether the results are normal or indicate a health issue.