The Serotonin test using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) is an advanced laboratory test designed to measure serotonin levels in biological samples, such as blood or urine. Serotonin is a neurotransmitter that plays an important role in regulating mood, sleep, and appetite, and has a significant impact on mental health. This technique is accurate and efficient in determining serotonin concentration, allowing for the analysis of complex compounds effectively. The test is used to assess mental disorders such as depression and anxiety, as well as to monitor certain medical conditions that affect serotonin levels. The results of this test help doctors guide appropriate treatment and gain a better understanding of the patient’s condition. This technique is also used in research to study the relationship between serotonin and various diseases, enhancing overall understanding of the role of this neurotransmitter in public health.