Melatonin, Plasma analysis by Liquid Chromatography Tandem Mass Spectrometry - LC-MS/MS, is used to accurately measure melatonin hormone levels in the blood. Melatonin is a natural hormone produced by the pineal gland and plays an important role in regulating the sleep-wake cycle. This analysis is specifically performed to evaluate sleep disorders, such as insomnia or jet lag, as well as to understand the impact of environmental factors on the biological clock. The liquid chromatography with tandem mass spectrometry (LC-MS/MS) technique provides high precision in measuring melatonin levels, which helps doctors develop appropriate treatment plans. Additionally, this analysis can be used in research to understand the effects of melatonin on general health and disease prevention.