Immunohistochemistry is used to detect the expression of Gross Cystic Disease Fluid Protein-15 (GCDFP-15), also known as Brst-2. This protein is naturally secreted by epithelial cells in the breast, sweat glands, and salivary glands. It is used as a highly specific auxiliary diagnostic marker to confirm the breast, sweat gland, or salivary origin of cancers, particularly in cases of metastasis or tumors of unknown origin.