Histamine Analysis using Liquid Chromatography Coupled with Tandem Mass Spectrometry (LC-MS/MS) is an advanced technique used to measure histamine levels in various biological samples, such as blood and urine. Histamine is a compound that plays a vital role in many physiological processes, including immune responses and sleep regulation. This method is characterized by its high accuracy and ability to detect very small amounts of the compound, making it ideal for clinical research and diagnostics. The process involves extracting samples, separating them using liquid chromatography, and then analyzing them with mass spectrometry to obtain precise results. Thanks to this technique, doctors and researchers can gain deeper insights into histamine levels and their health effects, aiding in the diagnosis of conditions such as allergies and inflammatory diseases.