Glutamine analysis using liquid chromatography–tandem mass spectrometry (LC-MS/MS) is an advanced test used to measure glutamine levels in biological fluids such as blood or plasma. Glutamine is an essential amino acid that plays a vital role in numerous biological processes, including protein synthesis, supporting immune function, and regulating nitrogen balance. This analysis is performed using a precise technique that combines liquid chromatography with mass spectrometry, providing accurate and sensitive measurements of glutamine levels. The test is used to evaluate various conditions, including metabolic disorders, anemia, liver diseases, and gastrointestinal disorders. The results help physicians identify changes in glutamine levels, guiding treatment plans and monitoring disease progression. This analysis is a valuable tool in modern medicine for better understanding metabolic health and nutritional balance.