Factor V Leiden Genetic Test - Factor V Leiden Mutation Test by PCR

Factor V Leiden mutation testing by polymerase chain reaction (PCR) is a molecular genetic analysis that detects a specific point mutation (adenine-guanine substitution at position 1691) in the factor V (F5) gene. This mutation produces an abnormal protein (Factor V Leiden) that resists inhibition by activated C protein, leading to hypercoagulability and an increased risk of venous thrombosis.