Catecholamines Fractionations, By High-Performance Liquid Chromatography (HPLC) analysis is a laboratory test used to measure levels of catecholamines, which are hormones that play a vital role in regulating the body’s responses to stress and pressure. The main catecholamines measured include dopamine, norepinephrine, and epinephrine. High-performance liquid chromatography (HPLC) is used to separate and analyze these compounds with high precision, allowing accurate evaluation of their levels in blood or urine. This analysis is considered an important tool in diagnosing a range of health conditions, such as neurogenic tumors (such as pheochromocytoma) and adrenal gland disorders, as well as monitoring the response to stress. This test is recommended when symptoms such as high blood pressure, increased heart rate, or anxiety- or stress-like symptoms appear, which may indicate disturbances in the catecholamine system. It provides valuable information for physicians in assessing the patient’s overall health status and guiding appropriate treatment.