The c-kit analysis is a laboratory test used to determine the presence and expression of the c-kit gene (also known as CD117) in cells. This gene is considered one of the vital genes that plays an important role in cell growth and development, and it is particularly expressed in some types of cancer cells. This analysis is mainly used in the diagnosis and monitoring of certain types of cancer, such as soft tissue sarcoma and leukemia. Determining the level of c-kit gene expression helps guide treatment options, as studies show that some targeted therapies are more effective in tumors that express this gene. The c-kit analysis is considered a valuable tool for physicians in evaluating patients’ health status and planning appropriate treatment.