The Androstenedione, Serum test is used to measure the level of the hormone androstenedione in the blood. This test helps evaluate the function of the adrenal glands and testes, and it can assist in diagnosing conditions such as endocrine disorders, infertility, and polycystic ovary syndrome (PCOS). A blood sample is typically taken in the morning, and it is important to follow the doctor's instructions for preparation before the test.