Amikacin serum testing is a quantitative measurement of the concentration of this aminoglycoside antibiotic in the blood. Amikacin is a potent bactericidal antibiotic used to treat severe infections caused by resistant gram-negative bacteria. Due to its narrow therapeutic range and potential nephrotoxicity (kidney toxicity) and ototoxicity (ear toxicity), careful monitoring of its serum levels (peak and trough) is essential for optimal and safe treatment.