Molecular test using Polymerase Chain Reaction (PCR) to detect DNA of Herpes Simplex Virus type I (HSV-1) and/or type II (HSV-2) in urine sample. Considered highly sensitive and specific test for diagnosing active infection, as it detects presence of the virus itself. Used especially for diagnosing herpes infection in urinary tract, or as non-invasive alternative for detecting systemic infection in newborns with suspected infection.