Biotin (B7) Analysis by High-Performance Liquid Chromatography (HPLC) is a laboratory procedure designed to determine biotin levels in biological samples such as blood or urine. This analysis is characterized by high accuracy and the ability to separate biotin from other compounds in the sample, allowing reliable assessment of an individual’s nutritional status. Liquid chromatography is used to identify precise biotin concentrations, which is important for hair, skin, and nail health, as well as its vital role in metabolic processes. This analysis is considered a valuable tool in medicine and nutrition, as it helps in the early detection of biotin deficiency and provides important data to guide appropriate treatments.