Vitamin B1 (Thiamine) analysis using High-Performance Liquid Chromatography (HPLC) is an important test used to measure thiamine levels in the blood. Thiamine is essential for the health of the nervous system and the metabolism of carbohydrates. A blood sample is collected and sent to the laboratory, where it is analyzed using HPLC technology, which allows for precise concentration measurements. This test is recommended in cases of vitamin deficiency or when symptoms such as persistent fatigue, poor concentration, or memory problems appear. The analysis helps doctors assess the patient's nutritional status and determine the need for supplements or dietary adjustments to ensure healthy thiamine levels.