Immunohistochemical assay for PAX5 (Paired Box 5) aims to detect this nuclear transcription factor, which is essential for the development and differentiation of B cells at all stages, from early precursors to plasma cells. PAX5 is a highly sensitive and specific marker of B cell origin, making it an essential tool in the diagnosis and classification of B-cell lymphomas and in differentiating them from T-cell lymphomas or non-lymphomas.