This diagnostic package, based on immunohistochemistry, includes the examination of a range of markers to evaluate and differentiate T cell subsets, particularly cytotoxic/suppressor T cells. The package primarily involves the examination of CD8, a membrane protein complex that acts as a co-receptor on the surface of these cells, along with essential complementary markers: CD3 (a general T cell marker), CD4 (a helper/regulatory T cell marker), and cytotoxicity markers such as Granzyme B or TIA-1. The package aims to determine the number, location, and function of CD8+ cells in tissues and to assess the balance between helper (CD4+) and cytotoxic (CD8+) cells.