Total Cortisol analysis using Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) is an advanced laboratory procedure used to measure cortisol hormone levels in blood or urine samples. Cortisol is a hormone secreted by the adrenal glands and plays a vital role in regulating various physiological functions, including the body's stress response, blood sugar control, and immune system support. The analysis process begins with collecting the sample, followed by careful preparation to separate cortisol from other components. Liquid chromatography is then used to separate the compounds, and mass spectrometry is employed to accurately measure the quantities. This test helps doctors diagnose a range of medical conditions, such as Cushing's syndrome, adrenal insufficiency, and certain psychiatric disorders. Thanks to its precision and reliability, the Total Cortisol analysis using LC-MS/MS is a crucial tool in modern healthcare, providing valuable insights into a patient's health status and stress response.