Dexamethasone analysis in serum or plasma by Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) is an advanced laboratory technique designed to measure dexamethasone levels, a corticosteroid used to treat a variety of medical conditions, including inflammation and immune-related diseases. The process begins with collecting a serum or plasma sample, which is then processed to remove unwanted compounds. Liquid chromatography is used to separate dexamethasone, followed by precise measurements using mass spectrometry to determine its concentration with high accuracy. This technique is reliable and rapid, providing accurate results that help physicians assess treatment effectiveness and adjust dosages. The analysis is also used in clinical research to evaluate the effects of dexamethasone in various health conditions.